Which of the following transitions has a distinct dividing line between two shots?

The transition that has a distinct dividing line between two shots is a wipe. In a wipe transition, one shot appears to push the other off the screen, creating a clear boundary or line where the two images meet. This technique clearly delineates the end of one scene and the beginning of another, making the transition visually obvious to the audience.

While other transitions like dissolves and cuts also signal changes between shots, they do so in different ways. A dissolve gradually mixes one shot into another, making the transition smooth and more subtle without a distinct line. A cut is an instantaneous change from one shot to another, which can also lack a noticeable dividing line. Establishing shots serve a different purpose by setting up the context of a scene but do not pertain directly to the method of transitioning between shots. Therefore, the wipe is identified as the transition method that distinctly marks the separation between shots.